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Wbc staining procedure

Staining Procedure of WBC WBC  or White blood cell is a special type of cell present in blood which helps to fight with bacteria, viruses and germs. There are mainly five types of WBC present in circulating blood. 1.Neutrophil.                   3.Lymphocyte 2.Eosinophil                    4.Monocyte         5.Basophil Discovery  Gabriel Andral and William Addison an English doctor both reported the first   descriptions of white blood cells. Staining Procedure of WBC for microscopic examination   1.Take a grease free slide. Put a drop of fresh blood on the slide. 2.With the help of another slide draw a thin smear. 3.Air dry the slide. 4.Pour few drops of Leishman's stain on the smear and allow it to reacts for 5 minutes. 5.After 5 minutes add little amount of distilled water into the stain, don't let it dry. Keep it for 30 minutes .The total time is depending upon the stain quality. 6.Next washed the slide in tap water and observed under the microscope.    
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Camera Lucida It is a device which is used for making  drawing of Microscopic animals.A prism is attached with the metal base.Its fits on the head of simple compound microscope.Camera Lucida(in Latin) meaning Light Chamber.This device performs a superimposition of the specimen which is viewed upon the surface upon which the scientist/students drawing.It is allows the students to draw a duplicate image of that specimen.This device was patented by Sir William Hyde Wollaston in the year of 1807.He was an English Chemist and Physicist.He also famous for discovering Palladium and Rhodium.

Spider eating Mosquito

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How to make Alkaline Iodide solution for Titration of dissolved Oxygen?

Take 50 grams of Sodium hydroxide and 15 gm of  Potassium/Sodium Iodide and dissolved in 100 ml of Distilled Water.

How to make Acid Fuchsin stain for laboratory use

How to make Acid Fuchsin stain for laboratory use? Take 10 percent Hcl (25 ml) and add 0.5-1 gm of Fuchsin powde r in it.Then add 300 ml distilled water in it.

How to make starch Solution for Laboratory Use?

How to make starch Solution for Laboratory Use? ANS : Weight 10 gm starch Powder .Dilute in 100 ml of distilled water and heat for 10 minutes at 80 degree centigrade. After heating cool it at room temperature and use for experiment.

Diagrammatic presentation of Cranial Nerve of Major Carp (Labeo rohita)

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