Staining Procedure of WBC
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WBC or White blood cell is a special type of cell present in blood which helps to fight with bacteria, viruses and germs. There are mainly five types of WBC present in circulating blood.
1.Neutrophil. 3.Lymphocyte
2.Eosinophil 4.Monocyte 5.Basophil
Discovery
Gabriel Andral and William Addison an English doctor both reported the first descriptions of white blood cells.
Staining Procedure of WBC for microscopic examination
1.Take a grease free slide. Put a drop of fresh blood on the slide.
2.With the help of another slide draw a thin smear.
3.Air dry the slide.
4.Pour few drops of Leishman's stain on the smear and allow it to reacts for 5 minutes.
5.After 5 minutes add little amount of distilled water into the stain, don't let it dry. Keep it for 30 minutes .The total time is depending upon the stain quality.
6.Next washed the slide in tap water and observed under the microscope.
WBC PERCENTAGE IN NORMAL BLOOD
NEUTROPHILS 62-65% EOSINOPHILS 2.5% LYMPHOCYTES 30%
MONOCYTES 5% BASOPHILS 1%
General characters of Neutrophil
1.They are Polymorphic. 2.Lifespan less than 24 hours in bloodstream.
3.Nucleus are multi lobed (2 to7lobes) 4.Approx 50% neutrophil attached with the
wall of blood vessels.
5.Presence of Granules.
Eosinophil
1.Bilobed nucleus(Headphone like shape).
2.Granules contains enzymes.
3.10-15µ in diameter.
4.Lifespan approx 8-12 hours in bloodstream.
Lymphocytes
1.They are two types-small and large. 3.Large lymphocyte contains more
2.They does not contains granules. cytoplasm than small lymphocyte.
4.Small lymphocyte contain less cytoplasm
and nucleus is dark coloured.
Monocyte
1.Nucleus Kidney shaped. 2.They have grey coloured cytoplasm.
3.Lifespan many days. 4. 15-19µ in diameter.
